Help

C7orf53 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
Overview
Catalog # bs-15273R-Cy5
Product Name C7orf53 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human C7orf53
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 286006
Subcellular location Cell membrane
Synonyms C7orf53; CG053_HUMAN; Chromosome 7 open reading frame 53; Coiled-coil domain-containing transmembrane protein C7orf53.
Background Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia. The C7orf53 gene product has been provisionally designated C7orf53 pending further characterization.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is C7orf53 Polyclonal Antibody, Cy5 Conjugated (bs-15273R-Cy5) validated for, and at what dilution?

bs-15273R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-15273R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C7orf53 Polyclonal Antibody, Cy5 Conjugated (bs-15273R-Cy5)?

KLH conjugated synthetic peptide derived from human C7orf53

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-15273R-Cy5)?

Cy5 on bs-15273R-Cy5: excitation / emission 625,650nm/670nm.