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C9orf85 Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-15345R-PerCP-Cy5.5-100uL
Product Name C9orf85 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Horse
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human C9orf85
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 66206
Swiss Prot Q96MD7
Subcellular location Nucleus
Synonyms C9orf85; Chromosome 9 open reading frame 85; CI085_HUMAN; Hypothetical protein LOC138241; MGC61599; OTTHUMP00000021459; OTTHUMP00000021460; Uncharacterized protein C9orf85.
Background Chromosome 9 consists of about 145 million bases and 4% of the human genome and encodes nearly 900 genes. Considered to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, is associated with the chromosome 9 gene encoding endoglin protein, ENG. Familial dysautonomia is also associated with chromosome 9 though through the gene IKBKAP. Notably, chromosome 9 encompasses the largest interferon family gene cluster. Chromosome 9 is partnered with chromosome 22 in the translocation leading to the aberrant production of BCR-ABL fusion protein often found in leukemias. The C9orf85 gene product has been provisionally designated C9orf85 pending further characterization. There are three isoforms of C9orf85 that are produced as a result of alternative splicing events.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is C9orf85 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15345R-PERCP-CY5-5) validated for, and at what dilution?

BS-15345R-PERCP-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does C9orf85 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15345R-PERCP-CY5-5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse.

How should BS-15345R-PERCP-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C9orf85 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15345R-PERCP-CY5-5)?

KLH conjugated synthetic peptide derived from human C9orf85

What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-15345R-PERCP-CY5-5)?

PerCP-Cy5.5 on BS-15345R-PERCP-CY5-5: excitation / emission 482nm/694nm.

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