Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody

$Mouse placenta lysates probed with AP-TNAP\/ALPL Polyclonal Antibody, unconjugated (bs-1535R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Mouse placenta lysates probed with AP-TNAP\/ALPL Polyclonal Antibody, unconjugated (bs-1535R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

$HepG2 cells probed with AP-TNAP\/ALPL Polyclonal Antibody, unconjugated (bs-1535R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)$

HepG2 cells probed with AP-TNAP\/ALPL Polyclonal Antibody, unconjugated (bs-1535R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Overview
Catalog #	bs-1535R
Product Name	Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from AP-TNAP/ALPL
Immunogen Range	52-150/524
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	11647
Swiss Prot	P09242
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	Akp2; TNAP; Akp-2; APTNAP; TNSALP; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; Alkaline phosphatase 2; Alkaline phosphatase liver/bone/kidney isozyme; Alpl
Background	Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200