Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody, RBITC Conjugated
Applications
| Overview | |
| Catalog # | bs-1535R-RBITC |
| Product Name | Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from AP-TNAP/ALPL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11647 |
| Swiss Prot | P09242 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Akp2; TNAP; Akp-2; APTNAP; TNSALP; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; Alkaline phosphatase 2; Alkaline phosphatase liver/bone/kidney isozyme; Alpl |
| Background | Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody, RBITC Conjugated (bs-1535R-RBITC) validated for, and at what dilution?
bs-1535R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1535R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Alkaline Phosphatase, Tissue Non-Specific isozyme Polyclonal Antibody, RBITC Conjugated (bs-1535R-RBITC)?
KLH conjugated synthetic peptide derived from AP-TNAP/ALPL
What are the excitation and emission wavelengths of the RBITC conjugate (bs-1535R-RBITC)?
RBITC on bs-1535R-RBITC: excitation / emission 540nm/625nm.