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HBLD2 Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-15417R-PerCP
Product Name HBLD2 Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Sheep, Horse, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HBLD2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms GK004; HESB-like domain-containing protein 2; hIscA; Iron sulfur assembly protein IscA; Iron-sulfur cluster assembly 1 homolog, mitochondrial; ISCA1; ISCA1_HUMAN.
Background ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions (Cozar-Castellano et al., 2004 [PubMed 15262227]).
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HBLD2 Polyclonal Antibody, PerCP Conjugated (bs-15417R-PerCP) validated for, and at what dilution?

bs-15417R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-15417R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HBLD2 Polyclonal Antibody, PerCP Conjugated (bs-15417R-PerCP)?

KLH conjugated synthetic peptide derived from human HBLD2

What are the excitation and emission wavelengths of the PerCP conjugate (bs-15417R-PerCP)?

PerCP on bs-15417R-PerCP: excitation / emission 482nm/677nm.