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HBS1L Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Pig
  • Rabbit
Overview
Catalog # bs-15420R
Product Name HBS1L Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Pig, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HBS1L
Immunogen Range 101-200/684
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10767
Subcellular location Cytoplasm
Synonyms EF 1a; ERF3 similar protein; ERFS; HBS1; HBS1 like protein; HBS1L; Hsp70 subfamily B suppressor 1 like protein; KIAA1038; HBS1L_HUMAN.
Background HBS1L is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and _-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HBS1L Polyclonal Antibody (bs-15420R) validated for, and at what dilution?

bs-15420R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HBS1L Polyclonal Antibody (bs-15420R) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Pig, Rabbit.

How should bs-15420R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HBS1L Polyclonal Antibody (bs-15420R)?

KLH conjugated synthetic peptide derived from human HBS1L Immunogen range: 101-200/684.