HDPTP Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-15434R-RBITC-100uL |
| Product Name | HDPTP Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Pig, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HDPTP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25930 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DKFZP564F0923; EC 3.1.3.48; HD PTP; HD-PTP; HDPTP; His domain containing protein tyrosine phosphatase; His domain protein tyrosine phosphatase; His domain-containing protein tyrosine phosphatase; KIAA1471; Protein tyrosine phosphatase non receptor type 23; protein tyrosine phosphatase non-receptor type 23; Protein tyrosine phosphatase TD 14; protein tyrosine phosphatase TD14; PTN23_HUMAN; PTP TD14; PTP-TD14; PTPN 23; PTPN23; Tyrosine protein phosphatase non receptor type 23; Tyrosine-protein phosphatase non-receptor type 23. |
| Background | HD-PTP is a 1,636 amino acid protein encoded by the human gene PTPN23. HD-PTP belongs to the protein-tyrosine phosphatase family, non-receptor class subfamily. It contains one BRO1 domain, two TPR repeats and one tyrosine-protein phosphatase domain. The C-terminal region contains the PTP-like domain, whereas the N-terminal region contains the two TPR regions. These regions are homologous to the yeast protein, BRO1, which is involved in the mitogen-activated protein kinase signaling pathway. Similarly, HD-PTP is believed to act as a negative regulator of Ras-mediated mitogenic activity and is phosphorylated upon DNA damage, probably by ATM or ATR. HD-PTP protein is differentially modulated by two angiogenic growth factors. While Vascular Endothelial Growth Factor (VEGF) has no affect on protein levels, Fibroblast Growth Factor-2 (FGF-2) induces HD-PTP degradation via the proteasome system. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HDPTP Polyclonal Antibody, RBITC Conjugated (bs-15434R-RBITC) validated for, and at what dilution?
bs-15434R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HDPTP Polyclonal Antibody, RBITC Conjugated (bs-15434R-RBITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig, Chicken.
How should bs-15434R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HDPTP Polyclonal Antibody, RBITC Conjugated (bs-15434R-RBITC)?
KLH conjugated synthetic peptide derived from human HDPTP
What are the excitation and emission wavelengths of the RBITC conjugate (bs-15434R-RBITC)?
RBITC on bs-15434R-RBITC: excitation / emission 540nm/625nm.