Help

heavy chain cardiac Myosin Polyclonal Antibody, PE Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-15444R-PE-100uL
Product Name heavy chain cardiac Myosin Polyclonal Antibody, PE Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MYH 6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4625
Subcellular location Cytoplasm
Synonyms Alpha MHC; ASD3; CMD1S; CMH1; MGC138376; MGC138378; MPD1; MYH 6; MYH 7; MYH6; MYH7; MYHC A; MYHC; MYHC B; MyHC-alpha; MyHC-beta; MYHCA; MYHCB; Myosin heavy chain cardiac muscle alpha isoform; Myosin heavy chain cardiac muscle beta isoform; Myosin heavy polypeptide 7 cardiac muscle beta; MYH6_HUMAN; MYH7_HUMAN.
Background Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.
Application Dilution
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is heavy chain cardiac Myosin Polyclonal Antibody, PE Conjugated (bs-15444R-PE) validated for, and at what dilution?

bs-15444R-PE is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does heavy chain cardiac Myosin Polyclonal Antibody, PE Conjugated (bs-15444R-PE) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-15444R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise heavy chain cardiac Myosin Polyclonal Antibody, PE Conjugated (bs-15444R-PE)?

KLH conjugated synthetic peptide derived from human MYH 6

What are the excitation and emission wavelengths of the PE conjugate (bs-15444R-PE)?

PE on bs-15444R-PE: excitation / emission 496,564nm/578nm.