HEMK2 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-15453R-BF647-100uL |
| Product Name | HEMK2 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HEMK2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29104 |
| Subcellular location | Cytoplasm |
| Synonyms | C21orf127; Chromosome 21 open reading frame 127; EC 2.1.1.-; HemK methyltransferase family member 2; HemK2; M.HsaHemK2P; MGC19995; MTQ2; N-6 adenine-specic DNA methyltransferase 1; N-6 adenine-specic DNA methyltransferase 1 putative; N6-DNA-methyltransferase; N6AMT; N6AMT1; OTTHUMP00000096393; OTTHUMP00000096394; PRED28; HEMK2_HUMAN. |
| Background | HEMK2 is a 214 amino acid protein that belongs to the methyltransferase superfamily and exists as multiple alternatively spliced isoforms. The gene encoding HEMK2 maps to human chromosome 21, which houses approximately 300 genes and comprises nearly 1.5% of the human genome. Chromosome 21-associated disorders include Alzheimer's disease, amyotrophic lateral sclerosis and, most notably, Down syndrome (also known as trisomy 21). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HEMK2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-15453R-BF647) validated for, and at what dilution?
bs-15453R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HEMK2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-15453R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Sheep, Horse.
How should bs-15453R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HEMK2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-15453R-BF647)?
KLH conjugated synthetic peptide derived from human HEMK2
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-15453R-BF647)?
AbBy Fluor-647 on bs-15453R-BF647: excitation / emission 650nm/665nm.