HERC3 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-15461R-PE-Cy5-100uL |
| Product Name | HERC3 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HERC3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8916 |
| Subcellular location | Cytoplasm |
| Synonyms | HECT domain and RCC1 like domain containing protein 3; Hect domain and RLD 3; HERC 3; KIAA0032; Probable E3 ubiquitin protein ligase HERC3; HERC3_HUMAN. |
| Background | HERC3 is a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is HERC3 Polyclonal Antibody, PE-Cy5 Conjugated (bs-15461R-PE-Cy5) validated for, and at what dilution?
bs-15461R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-15461R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HERC3 Polyclonal Antibody, PE-Cy5 Conjugated (bs-15461R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human HERC3
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-15461R-PE-Cy5)?
PE-Cy5 on bs-15461R-PE-Cy5: excitation / emission 488nm/670nm.