ERVFRD-1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-15466R-RBITC-100uL |
| Product Name | ERVFRD-1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Syncytin-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 405754 |
| Swiss Prot | P60508 |
| Subcellular location | Cell membrane |
| Synonyms | envFRD; UNQ6191; ERVFRDE1; GLLL6191; HERV-FRD; HERV-W/FRD; Syncytin-2; Endogenous retrovirus group FRD member 1; Envelope polyprotein; HERV-FRD_6p24.1 provirus ancestral Env polyprotein; ERVFRD-1; UNQ6191/PRO20218 |
| Background | This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process (PubMed:18988732). Endogenous envelope proteins may have kept, lost or modified their original function during evolution but this one can still make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein mediates receptor recognition, while the transmembrane protein anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane (PubMed:14694139). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ERVFRD-1 Polyclonal Antibody, RBITC Conjugated (bs-15466R-RBITC) validated for, and at what dilution?
bs-15466R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ERVFRD-1 Polyclonal Antibody, RBITC Conjugated (bs-15466R-RBITC) react with?
Tested reactivity: Human.
How should bs-15466R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ERVFRD-1 Polyclonal Antibody, RBITC Conjugated (bs-15466R-RBITC)?
KLH conjugated synthetic peptide derived from human Syncytin-2
What are the excitation and emission wavelengths of the RBITC conjugate (bs-15466R-RBITC)?
RBITC on bs-15466R-RBITC: excitation / emission 540nm/625nm.