Hho1 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-15480R-Cy5-100uL |
| Product Name | Hho1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | yeast |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae Hho1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Hho1p; Histone H1, a linker histone required for nucleosome packaging at restricted sites; LPI17C; Not required for telomeric silencing, basal transcriptional repression, or efficient sporulation; Suppresses DNA repair involving homologous recombination; YPL127C; H1_YEAST. |
| Background | Hho1 |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Hho1 Polyclonal Antibody, Cy5 Conjugated (bs-15480R-Cy5) validated for, and at what dilution?
bs-15480R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-15480R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hho1 Polyclonal Antibody, Cy5 Conjugated (bs-15480R-Cy5)?
KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae Hho1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-15480R-Cy5)?
Cy5 on bs-15480R-Cy5: excitation / emission 625,650nm/670nm.