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FOXO3A Polyclonal Antibody

L1 Rat brain lysate L2 human colon carcinoma 30ug, probed (bs-1548R) at 1:200 in 4\u02daC. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 74kDa.
L1 Rat brain lysate L2 human colon carcinoma 30ug, probed (bs-1548R) at 1:200 in 4\u02daC. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 74kDa.
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nFOXO3A Polyclonal Antibody(bs-1548R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nFOXO3A Polyclonal Antibody(bs-1548R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human DU145 cell lysates probed with FOXO3A Polyclonal Antibody, Unconjugated (bs-1548R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human DU145 cell lysates probed with FOXO3A Polyclonal Antibody, Unconjugated (bs-1548R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
25 ug total protein per lane of various lysates (see on figure) probed with FOXO3A polyclonal antibody, unconjugated (bs-1548R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with FOXO3A polyclonal antibody, unconjugated (bs-1548R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-1548R
Product Name FOXO3A Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FOXO3A
Immunogen Range 581-673/673
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2309
Swiss Prot O43524
Subcellular location Cytoplasm, Nucleus
Synonyms AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; 1110048B16Rik; 2010203A17Rik; Fkhr2; FOXO3_HUMAN; FOXO3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3_MOUSE; forkhead box O3
Background Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F IFICC1:50-200
IF

Frequently asked questions

Which applications is FOXO3A Polyclonal Antibody (bs-1548R) validated for, and at what dilution?

bs-1548R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FOXO3A Polyclonal Antibody (bs-1548R) react with?

Tested reactivity: Human, Mouse.

How should bs-1548R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FOXO3A Polyclonal Antibody (bs-1548R)?

KLH conjugated synthetic peptide derived from human FOXO3A Immunogen range: 581-673/673.

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