phospho-ICK (Tyr159) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-15535R-BF405 |
| Product Name | phospho-ICK (Tyr159) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ICK(Tyr159) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ICKTyr159; 2210420N10Rik; AI848300; ECO; ICK_HUMAN; Heart serine threonine protein kinase; hICK; Intestinal cell MAK like kinase; Intestinal cell kinase; KIAA0936; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MAK related kinase; MGC46090; mICK; MRK; OTTMUSP00000041856; OTTMUSP00000041857; Serine/threonine-protein kinase ICK; Serine/threonine protein kinase ICK. |
| Background | Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ICK (Tyr159) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-15535R-BF405) validated for, and at what dilution?
bs-15535R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-15535R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ICK (Tyr159) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-15535R-BF405)?
KLH conjugated synthetic peptide derived from human ICK(Tyr159)
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-15535R-BF405)?
AbBy Fluor-405 on bs-15535R-BF405: excitation / emission 401nm/421nm.