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IDN3 Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-15541R-PerCP
Product Name IDN3 Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IDN3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Nucleus
Synonyms CDLS; Colon tumor susceptibility 2; Delangin; DKFZp434L1319; FLJ11203; FLJ12597; FLJ13354; FLJ13648; FLJ44854; IDN 3; IDN 3 protein; IDN 3 protein isoform A; IDN 3 protein isoform B; IDN 3B; IDN3 B; IDN3 protein; IDN3 protein isoform A; IDN3 protein isoform B; IDN3B; Mis 4; Mis4; Nipbl; NIPBL_HUMAN; Nipped B homolog Drosophila; Nipped B homolog; Nipped B like; Nipped B like protein; Nipped-B-like protein; Scc 2; SCC 2 homolog; Scc2; SCC2 homolog; Sister chromatid cohesion protein Mis4.
Background This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IDN3 Polyclonal Antibody, PerCP Conjugated (bs-15541R-PerCP) validated for, and at what dilution?

bs-15541R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IDN3 Polyclonal Antibody, PerCP Conjugated (bs-15541R-PerCP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-15541R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IDN3 Polyclonal Antibody, PerCP Conjugated (bs-15541R-PerCP)?

KLH conjugated synthetic peptide derived from human IDN3

What are the excitation and emission wavelengths of the PerCP conjugate (bs-15541R-PerCP)?

PerCP on bs-15541R-PerCP: excitation / emission 482nm/677nm.