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Aquaporin 5 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-1554R-Cy3-100uL
Product Name Aquaporin 5 Polyclonal Antibody, Cy3 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse AQP5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11830
Swiss Prot Q9WTY4
Subcellular location Cell membrane
Synonyms Aquaporin-5; AQP-5; Aqp5
Background Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Aquaporin 5 Polyclonal Antibody, Cy3 Conjugated (bs-1554R-Cy3) validated for, and at what dilution?

bs-1554R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Aquaporin 5 Polyclonal Antibody, Cy3 Conjugated (bs-1554R-Cy3) react with?

Tested reactivity: Mouse, Rat.

How should bs-1554R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Aquaporin 5 Polyclonal Antibody, Cy3 Conjugated (bs-1554R-Cy3)?

KLH conjugated synthetic peptide derived from mouse AQP5

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1554R-Cy3)?

Cy3 on bs-1554R-Cy3: excitation / emission 512,550nm/570,615nm.

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