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IGLL1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
Overview
Catalog # bs-15577R-PerCP-Cy5.5
Product Name IGLL1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IGLL1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Secreted
Synonyms 14.1; AGM2; CD179 antigen-like family member B; CD179B; CD179b antigen; Ig lambda 5; Ig lambda-5; IGL1; IGL5; IGLJ14.1; IGLL; Igll1; IGLL1_HUMAN; IGO; IGVPB; Immunoglobulin lambda like polypeptide 1 precursor; Immunoglobulin lambda-like polypeptide 1; Immunoglobulin omega polypeptide; Immunoglobulin omega polypeptide chain; Immunoglobulin related protein 14.1; Immunoglobulin-related protein 14.1; Lambda5; Pre B lymphocyte specic protein 2; VPREB2.
Background The preB cell receptor is found on the surface of proB and preB cells, where it is involved in transduction of signals for cellular proliferation, differentiation from the proB cell to the preB cell stage, allelic exclusion at the Ig heavy chain gene locus, and promotion of Ig light chain gene rearrangements. The preB cell receptor is composed of a membrane-bound Ig mu heavy chain in association with a heterodimeric surrogate light chain. This gene encodes one of the surrogate light chain subunits and is a member of the immunoglobulin gene superfamily. This gene does not undergo rearrangement. Mutations in this gene can result in B cell deficiency and agammaglobulinemia, an autosomal recessive disease in which few or no gamma globulins or are made. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IGLL1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15577R-PERCP-CY5-5) validated for, and at what dilution?

BS-15577R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IGLL1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15577R-PERCP-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should BS-15577R-PERCP-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IGLL1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15577R-PERCP-CY5-5)?

KLH conjugated synthetic peptide derived from human IGLL1

What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-15577R-PERCP-CY5-5)?

PerCP-Cy5.5 on BS-15577R-PERCP-CY5-5: excitation / emission 482nm/694nm.