HBsAg Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1557g-cy5.5-100uL |
| Product Name | HBsAg Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Virus |
| Predicted Reactivity | Bee |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Goat |
| Source | Human Hepatitis B Surface Antigen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | Q67926 |
| Subcellular location | Cell membrane |
| Synonyms | HBsAg; HBV major surface antigen; HBV surface antigen; Hepatitis B Virus major surface antigen; Major surface antigen; S; Human Hepatitis B Surface Antigen |
| Background | Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HBsAg Polyclonal Antibody, Cy5.5 Conjugated (BS-1557G-CY5-5) validated for, and at what dilution?
BS-1557G-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HBsAg Polyclonal Antibody, Cy5.5 Conjugated (BS-1557G-CY5-5) react with?
Tested reactivity: Human, Virus. Predicted reactivity (expected from sequence homology but not yet tested): Bee.
How should BS-1557G-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HBsAg Polyclonal Antibody, Cy5.5 Conjugated (BS-1557G-CY5-5)?
Human Hepatitis B Surface Antigen
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-1557G-CY5-5)?
Cy5.5 on BS-1557G-CY5-5: excitation / emission 675nm/694nm.