phospho-IKB epsilon (Ser22) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-15582R-BF488 |
| Product Name | phospho-IKB epsilon (Ser22) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human IKB epsilon around the phosphorylation site of Ser22 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | I kappa B epsilon; I-kappa-B-epsilon; IkappaBepsilon; IkB E; IkB-E; IkB-epsilon; IKBE; IKBE_MOUSE; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFkappa BIE; NFkappaB inhibitor epsilon; NFKBIE; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon; OTTHUMP00000016522; Slc35b2; solute carrier family 35, member B2. |
| Background | IKB epsilon (Ser22) |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IKB epsilon (Ser22) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-15582R-BF488) validated for, and at what dilution?
bs-15582R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IKB epsilon (Ser22) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-15582R-BF488) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep.
How should bs-15582R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IKB epsilon (Ser22) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-15582R-BF488)?
KLH conjugated synthesised phosphopeptide derived from human IKB epsilon around the phosphorylation site of Ser22
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-15582R-BF488)?
AbBy Fluor-488 on bs-15582R-BF488: excitation / emission 499nm/519nm.