TEM8 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-15583R-RBITC |
| Product Name | TEM8 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TEM8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84168 |
| Swiss Prot | Q9H6X2 |
| Subcellular location | Cell membrane |
| Synonyms | Anthrax toxin receptor 1; Anthrax toxin receptor 1 precursor; ANTXR 1; ANTXR1; ATR; TEM-8; TEM 8; TEM8; Tumor Endothelial Marker 8; Tumor endothelial marker 8 precursor; ANTR1_HUMAN; Anthrax toxin receptor-1; FLJ11298; FLJ21776. |
| Background | This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TEM8 Polyclonal Antibody, RBITC Conjugated (bs-15583R-RBITC) validated for, and at what dilution?
bs-15583R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TEM8 Polyclonal Antibody, RBITC Conjugated (bs-15583R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Rabbit.
How should bs-15583R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TEM8 Polyclonal Antibody, RBITC Conjugated (bs-15583R-RBITC)?
KLH conjugated synthetic peptide derived from human TEM8
What are the excitation and emission wavelengths of the RBITC conjugate (bs-15583R-RBITC)?
RBITC on bs-15583R-RBITC: excitation / emission 540nm/625nm.