Collagen VII Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-1558R-Cy3-100uL |
| Product Name | Collagen VII Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Collagen VII |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1294 |
| Swiss Prot | Q02388 |
| Subcellular location | Extracellular matrix |
| Synonyms | COL7A1; Collagen alpha-1(VII) chain; Long-chain collagen; LC collagen; collagen type VII alpha 1 chain; EBD1; EBR1; EBDCT; |
| Background | Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. |
| Application Dilution | |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Collagen VII Polyclonal Antibody, Cy3 Conjugated (bs-1558R-Cy3) validated for, and at what dilution?
bs-1558R-Cy3 is validated for IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1558R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen VII Polyclonal Antibody, Cy3 Conjugated (bs-1558R-Cy3)?
KLH conjugated synthetic peptide derived from human Collagen VII
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1558R-Cy3)?
Cy3 on bs-1558R-Cy3: excitation / emission 512,550nm/570,615nm.