NFIL3 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-15592r-hrp |
| Product Name | NFIL3 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NFIL3 |
| Immunogen Range | 21-120/462 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | E4 promoter binding protein 4; E4 promoter-binding protein 4; E4BP 4; E4BP4; E4BP4 protein; IL3BP 1; IL3BP1; Interleukin 3 binding protein 1; Interleukin 3 promoter transcriptional activator; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL 3; NFIL 3A; NFIL3; NFIL3_HUMAN; NFIL3A; Nuclear factor interleukin 3 regulated; Nuclear factor interleukin-3-regulated protein; Transcriptional activator NF IL3A; Transcriptional activator NF-IL3A. |
| Background | Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM, Feb 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is NFIL3 Polyclonal Antibody, HRP Conjugated (bs-15592R-HRP) validated for, and at what dilution?
bs-15592R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NFIL3 Polyclonal Antibody, HRP Conjugated (bs-15592R-HRP) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep.
How should bs-15592R-HRP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NFIL3 Polyclonal Antibody, HRP Conjugated (bs-15592R-HRP)?
KLH conjugated synthetic peptide derived from human NFIL3 Immunogen range: 21-120/462.