F-Actin Polyclonal Antibody

Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 42kDa

L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 42kDa

A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat
Dog
Chicken

Predicted Reactivity

Cow
Sheep
Pig

Overview
Catalog #	bs-1571R
Product Name	F-Actin Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat, Dog, Chicken
Predicted Reactivity	Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit
Immunogen Range	1-50/374
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	60
Swiss Prot	P52907
Subcellular location	Cytoplasm
Synonyms	CapZ alpha-1; F-actin-capping protein subunit alpha-1; CAZA1_HUMAN
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

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