ATP7A Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1572R-BF750 |
| Product Name | ATP7A Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATP7A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 538 |
| Swiss Prot | Q04656 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | MK; MNK; DSMAX; SMAX3; Copper-transporting ATPase 1; Copper pump 1; Menkes disease-associated protein; ATP7A; MC1 |
| Background | May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ATP7A Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-1572R-BF750) validated for, and at what dilution?
bs-1572R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATP7A Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-1572R-BF750) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Rabbit.
How should bs-1572R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATP7A Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-1572R-BF750)?
KLH conjugated synthetic peptide derived from human ATP7A
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-1572R-BF750)?
AbBy Fluor-750 on bs-1572R-BF750: excitation / emission 749nm/775nm.