IFNA13 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Brain Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IFNA8 Polyclonal Antibody, Unconjugated (bs-1578R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human Brain Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IFNA8 Polyclonal Antibody, Unconjugated (bs-1578R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-1578R
Product Name	IFNA13 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody is specific for Human IFNA8 and does not cross-react with other IFN-Alpha subtypes
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IFNA8
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3445
Swiss Prot	P32881
Subcellular location	Secreted
Synonyms	Interferon alpha-8; IFN-alpha-8; IFN-alphaB; Interferon alpha-B; LeIF B; Interferon alpha-B2
Background	Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200