SFTPD Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse lung tissue labeled Anti-SP-D Polyclonal Antibody, Unconjugated (bs-1583R) at 1:2000, followed by conjugation to the secondary antibody and DAB staining

THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SP-D\/PSPD Antibody(bs-1583R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).