alpha Adducin(Ser436) Antibody

$Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha Adducin(Ser436) Polyclonal Antibody, Unconjugated (bs-15968R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha Adducin(Ser436) Polyclonal Antibody, Unconjugated (bs-15968R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Lane 1: Mouse Testis lysates; Lane 2: Mouse Spleen lysates; Lane 3: Molt-4 cell lysates probed with alpha Adducin(Ser436) Polyclonal Antibody, Unconjugated (bs-15968R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Horse
Chicken

Overview
Catalog #	bs-15968R
Product Name	alpha Adducin(Ser436) Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of Ser436
Modification Site	Ser436
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	118
Subcellular location	Cytoplasm, Cell membrane
Synonyms	alpha Adducinphospho S436; alpha Adducinphospho Ser436; p-alpha AdducinS436; p-alpha AdducinSer436; ADD 1; ADD A; ADD1; ADD1 protein; ADDA; ADDA_HUMAN; Adducin 1 alpha; Adducin 1 alpha; Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte hosphor subunit alpha; Erythrocyte adducin subunit alpha.
Background	Adducins are a family of cytoskeleton proteins encoded by three genes (Alpha, Beta, Gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin Alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin Alpha and Adducin Gamma is ubiquitous in contrast to the restricted expression of Adducin Beta. Adducin Beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

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