phospho-alpha Adducin (Ser436) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-15968R-BF750 |
| Product Name | phospho-alpha Adducin (Ser436) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of Ser436 |
| Modification Site | Ser436 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 118 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | alpha Adducinphospho S436; alpha Adducinphospho Ser436; p-alpha AdducinS436; p-alpha AdducinSer436; ADD 1; ADD A; ADD1; ADD1 protein; ADDA; ADDA_HUMAN; Adducin 1 alpha; Adducin 1 alpha; Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte hosphor subunit alpha; Erythrocyte adducin subunit alpha. |
| Background | Adducins are a family of cytoskeleton proteins encoded by three genes (Alpha, Beta, Gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin Alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin Alpha and Adducin Gamma is ubiquitous in contrast to the restricted expression of Adducin Beta. Adducin Beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-alpha Adducin (Ser436) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-15968R-BF750) validated for, and at what dilution?
bs-15968R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-alpha Adducin (Ser436) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-15968R-BF750) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Chicken.
How should bs-15968R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-alpha Adducin (Ser436) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-15968R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of Ser436
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-15968R-BF750)?
AbBy Fluor-750 on bs-15968R-BF750: excitation / emission 749nm/775nm.