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HRPT2 Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-1597R-PerCP
Product Name HRPT2 Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Sheep, Pig
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HRPT2/Parafibromin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 79577
Subcellular location Nucleus
Synonyms C1orf28;CDC 73;CDC73;Cell division cycle 73;Cell division cycle 73 Paf1/RNA polymerase II complex component homolog;Cell division cycle protein 73 homolog;FLJ23316;HPT-JT;HRPT 2;Hyperparathyroidism 2 with jaw tumor;Hyperparathyroidism 2;Hyperparathyroidism 2 protein;Parafibromin.
Background The HRPT2 gene product, parafibromin, is a tumor suppressor protein that is part of the human Paf1 complex. The yeast counterpart to this complex is part of the RNA polymerase II complex as well, and is important for histone modification and connections to posttranscriptional events. Human parafibromin also associates with the RNA polymerase II large subunit.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HRPT2 Polyclonal Antibody, PerCP Conjugated (bs-1597R-PerCP) validated for, and at what dilution?

bs-1597R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HRPT2 Polyclonal Antibody, PerCP Conjugated (bs-1597R-PerCP) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig.

How should bs-1597R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HRPT2 Polyclonal Antibody, PerCP Conjugated (bs-1597R-PerCP)?

KLH conjugated synthetic peptide derived from human HRPT2/Parafibromin

What are the excitation and emission wavelengths of the PerCP conjugate (bs-1597R-PerCP)?

PerCP on bs-1597R-PerCP: excitation / emission 482nm/677nm.