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Cathepsin G Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-1598R-Cy3
Product Name Cathepsin G Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cathepsin G
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1511
Subcellular location Nucleus, Cell membrane
Synonyms Cathepsin G; CG; Cathepsin G precursor; CathepsinG; CTSG; CTSG protein; MGC23078; CATG_HUMAN.
Background Cathepsin G is a serine protease, it consisting of a single polypeptide with a molecular weight of 30 kDa as determined by SDS-PAGE. Its substrates are collagen and proteoglycans. Poor regulation of Cathepsin G levels has been associated with various connective tissue disorders, for example pulmonary emphysema and cystic fibrosis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Cathepsin G Polyclonal Antibody, Cy3 Conjugated (bs-1598R-Cy3) validated for, and at what dilution?

bs-1598R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cathepsin G Polyclonal Antibody, Cy3 Conjugated (bs-1598R-Cy3) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Horse, Rabbit.

How should bs-1598R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cathepsin G Polyclonal Antibody, Cy3 Conjugated (bs-1598R-Cy3)?

KLH conjugated synthetic peptide derived from human Cathepsin G

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1598R-Cy3)?

Cy3 on bs-1598R-Cy3: excitation / emission 512,550nm/570,615nm.