Help

FIBIN Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-16085R-FITC-100uL
Product Name FIBIN Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FIBIN
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 387758
Swiss Prot Q8TAL6
Subcellular location Cytoplasm
Synonyms Fin bud initiation factor homolog; FIBIN; PSEC0235
Background FIBIN (Fin bud initiation factor homolog) is a 211 amino acid protein involved in fin initiation in zebrafish. The human homolog is encoded by a gene that maps to chromosome 11, which makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. The chromosome 11 encoded Atm gene is important for regulation of cell cycle arrest and apoptosis following double strand DNA breaks. Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and _ thalassemia are caused by HBB gene mutations. Wilms' tumors, WAGR syndrome and Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are also associated with defects in chromosome 11.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FIBIN Polyclonal Antibody, FITC Conjugated (bs-16085R-FITC) validated for, and at what dilution?

bs-16085R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FIBIN Polyclonal Antibody, FITC Conjugated (bs-16085R-FITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-16085R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FIBIN Polyclonal Antibody, FITC Conjugated (bs-16085R-FITC)?

KLH conjugated synthetic peptide derived from human FIBIN

What are the excitation and emission wavelengths of the FITC conjugate (bs-16085R-FITC)?

FITC on bs-16085R-FITC: excitation / emission 494nm/518nm.