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CDH17_LI Cadherin Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti LI-cadherin Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti LI-cadherin Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti-LI-cadherin Polyclonal Antibody, Unconjugated (bs-1617R) followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti-LI-cadherin Polyclonal Antibody, Unconjugated (bs-1617R) followed by conjugation to the secondary antibody and DAB staining\\n
SW480 lysates probed with LI-cadherin Polyclonal Antibody, Unconjugated (bs-1617R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC
SW480 lysates probed with LI-cadherin Polyclonal Antibody, Unconjugated (bs-1617R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC
Lane 1: Human FHC cell Lysates; Lane 2: Human LOVO cell Lysates; Lane 3: Human SW480 cell Lysates. Probed with LI Cadherin polyclonal Antibody, unconjugated (bs-1617R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human FHC cell Lysates; Lane 2: Human LOVO cell Lysates; Lane 3: Human SW480 cell Lysates. Probed with LI Cadherin polyclonal Antibody, unconjugated (bs-1617R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (bs-1617R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-1617R
Product Name CDH17_LI Cadherin Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LI-cadherin
Immunogen Range 101-200/832
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1015
Swiss Prot Q12864
Subcellular location Cell membrane
Synonyms HPT1; CDH16; HPT-1; Cadherin-17; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin; LI-cadherin; CDH17
Background Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F IHC-P1:200-400
IF

Frequently asked questions

Which applications is CDH17_LI Cadherin Polyclonal Antibody (bs-1617R) validated for, and at what dilution?

bs-1617R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDH17_LI Cadherin Polyclonal Antibody (bs-1617R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-1617R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDH17_LI Cadherin Polyclonal Antibody (bs-1617R)?

KLH conjugated synthetic peptide derived from human LI-cadherin Immunogen range: 101-200/832.

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