GLIS3 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-16249r-100uL |
| Product Name | GLIS3 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Rat, Sheep |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GLIS3 |
| Immunogen Range | 101-200/775 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 169792 |
| Swiss Prot | Q8NEA6 |
| Subcellular location | Nucleus |
| Synonyms | NDH; ZNF515; GLIS3_HUMAN; GLIS3; GLI-similar 3; Zinc finger protein 515; GLIS3_MOUSE; GLIS family zinc finger 3 |
| Background | Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity). |
| Application Dilution | |
| WB | =1:500-2000 |
| ELISA | ELISA=1:5000-10000 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:100-500 |
Frequently asked questions
Which applications is GLIS3 Polyclonal Antibody (bs-16249R) validated for, and at what dilution?
bs-16249R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GLIS3 Polyclonal Antibody (bs-16249R) react with?
Tested reactivity: Human, Rat, Sheep.
How should bs-16249R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GLIS3 Polyclonal Antibody (bs-16249R)?
KLH conjugated synthetic peptide derived from human GLIS3 Immunogen range: 101-200/775.