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GPN3 Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-16278R-BF647
Product Name GPN3 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GPN3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51184
Swiss Prot Q9UHW5
Subcellular location Cytoplasm, Nucleus
Synonyms ATP-binding domain 1 family member C; ATPBD1C; GPN-loop GTPase 3; Gpn3; GPN3_HUMAN.
Background Small GTPase required for proper localization of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GPN3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16278R-BF647) validated for, and at what dilution?

bs-16278R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GPN3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16278R-BF647) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken.

How should bs-16278R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GPN3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16278R-BF647)?

KLH conjugated synthetic peptide derived from human GPN3

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-16278R-BF647)?

AbBy Fluor-647 on bs-16278R-BF647: excitation / emission 650nm/665nm.