B3GAT1 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1635R-BF555 |
| Product Name | B3GAT1 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human B3GAT1/CD57 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27087 |
| Swiss Prot | Q9P2W7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P; B3GAT1 |
| Background | Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is B3GAT1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-1635R-BF555) validated for, and at what dilution?
bs-1635R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does B3GAT1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-1635R-BF555) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Chicken.
How should bs-1635R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise B3GAT1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-1635R-BF555)?
KLH conjugated synthetic peptide derived from human B3GAT1/CD57
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-1635R-BF555)?
AbBy Fluor-555 on bs-1635R-BF555: excitation / emission 553nm/568nm.