phospho-JNK1 + 2 + 3 (Thr183+Tyr185) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1640R-RBITC-100uL |
| Product Name | phospho-JNK1 + 2 + 3 (Thr183+Tyr185) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185 |
| Modification Site | THR183+TYR185 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5599 |
| Swiss Prot | P45983 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK8 |
| Background | JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-JNK1 + 2 + 3 (Thr183+Tyr185) Polyclonal Antibody, RBITC Conjugated (bs-1640R-RBITC) validated for, and at what dilution?
bs-1640R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-JNK1 + 2 + 3 (Thr183+Tyr185) Polyclonal Antibody, RBITC Conjugated (bs-1640R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig.
How should bs-1640R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-JNK1 + 2 + 3 (Thr183+Tyr185) Polyclonal Antibody, RBITC Conjugated (bs-1640R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185
What are the excitation and emission wavelengths of the RBITC conjugate (bs-1640R-RBITC)?
RBITC on bs-1640R-RBITC: excitation / emission 540nm/625nm.