phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1646R-BF350 |
| Product Name | phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185 |
| Modification Site | Thr183/Tyr185 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55945595 |
| Swiss Prot | P28482 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ERK; p38; p4; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; Extracellular signal-regulated kinase 2; MAP kinase isoform p42; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1 |
| Background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1646R-BF350) validated for, and at what dilution?
bs-1646R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1646R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-1646R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1646R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-1646R-BF350)?
AbBy Fluor-350 on bs-1646R-BF350: excitation / emission 346nm/442nm.