HSF1 (Thr142) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-16678r-cy5-100uL |
| Product Name | HSF1 (Thr142) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Thr142 |
| Modification Site | Thr142 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3297 |
| Swiss Prot | Q00613 |
| Synonyms | HSF1 (phospho T142); p-HSF1 (phospho T142); Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSF1_HUMAN; HSTF 1; HSTF1. |
| Background | The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is HSF1 (Thr142) Polyclonal Antibody, Cy5 Conjugated (bs-16678R-Cy5) validated for, and at what dilution?
bs-16678R-Cy5 is validated for IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSF1 (Thr142) Polyclonal Antibody, Cy5 Conjugated (bs-16678R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-16678R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSF1 (Thr142) Polyclonal Antibody, Cy5 Conjugated (bs-16678R-Cy5)?
KLH conjugated synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Thr142
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-16678R-Cy5)?
Cy5 on bs-16678R-Cy5: excitation / emission 625,650nm/670nm.