phospho-HEF1 (Ser369) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16681R-BF405 |
| Product Name | phospho-HEF1 (Ser369) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human HEF1 around the phosphorylation site of Ser369 |
| Modification Site | Ser369 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4739 |
| Swiss Prot | Q14511 |
| Subcellular location | Cytoplasm |
| Synonyms | HEF1 (phospho S369); p-HEF1 (phospho S369); Cas like docking; Cas scaffolding protein family member 2; CAS-L; CAS2; CasL; CASL_HUMAN; CASS2; Crk associated substrate related; Crk associated substrate related protein; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ49G10.2; dJ761I2.1 (enhancer of filamentation (HEF1)); dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF 1; HEF1 |
| Background | The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-HEF1 (Ser369) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-16681R-BF405) validated for, and at what dilution?
bs-16681R-BF405 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HEF1 (Ser369) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-16681R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Horse.
How should bs-16681R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HEF1 (Ser369) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-16681R-BF405)?
KLH conjugated synthesised phosphopeptide derived from human HEF1 around the phosphorylation site of Ser369
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-16681R-BF405)?
AbBy Fluor-405 on bs-16681R-BF405: excitation / emission 401nm/421nm.