phospho-GRK2 (Ser670) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16682R-HRP-100uL |
| Product Name | phospho-GRK2 (Ser670) Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human GRK2 around the phosphorylation site of Ser670 |
| Modification Site | Ser670 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 156 |
| Swiss Prot | P25098 |
| Subcellular location | Cytoplasm |
| Synonyms | GRK2 (phospho S670); p-GRK2 (phospho S670); G-protein coupled receptor kinase 2; ADRBK 1; ADRBK1; Adrenergic beta receptor kinase 1; BARK 1; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; G Protein Coupled Receptor Kinase 2; G protein dependent receptor kinase 2; FLJ16718; GRK 2; ARBK1_HUMAN. |
| Background | The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-GRK2 (Ser670) Polyclonal Antibody, HRP Conjugated (bs-16682R-HRP) validated for, and at what dilution?
bs-16682R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GRK2 (Ser670) Polyclonal Antibody, HRP Conjugated (bs-16682R-HRP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-16682R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GRK2 (Ser670) Polyclonal Antibody, HRP Conjugated (bs-16682R-HRP)?
KLH conjugated synthesised phosphopeptide derived from human GRK2 around the phosphorylation site of Ser670