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IRAK1BP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Rabbit
Overview
Catalog # bs-16694R-BF647
Product Name IRAK1BP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody may exhibit a minor secondary cross-reactivity with SMPD5 due to a 63% contiguous sequence similarity over the immunogen range.
Reactivity Human, Mouse
Predicted Reactivity Rat, Rabbit
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IRAK1BP1.
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 134728
Swiss Prot Q5VVH5
Subcellular location Cytoplasm, Nucleus
Synonyms AIP70; SIMPL; Interleukin-1 receptor-associated kinase 1-binding protein 1; IRAK1-binding protein 1; IRAK1BP1
Background Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. Acts by enhancing RELA transcriptional activity (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IRAK1BP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16694R-BF647) validated for, and at what dilution?

bs-16694R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IRAK1BP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16694R-BF647) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Rabbit.

How should bs-16694R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IRAK1BP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16694R-BF647)?

KLH conjugated synthetic peptide derived from human IRAK1BP1.

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-16694R-BF647)?

AbBy Fluor-647 on bs-16694R-BF647: excitation / emission 650nm/665nm.