phospho-IRE1 (Ser724) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16698R-Cy5.5-100uL |
| Product Name | phospho-IRE1 (Ser724) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724 |
| Modification Site | Ser724 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10595 |
| Swiss Prot | O75460 |
| Synonyms | IRE1 (phospho S724); p-IRE1 (phospho S724); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1. |
| Background | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is phospho-IRE1 (Ser724) Polyclonal Antibody, Cy5.5 Conjugated (BS-16698R-CY5-5) validated for, and at what dilution?
BS-16698R-CY5-5 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IRE1 (Ser724) Polyclonal Antibody, Cy5.5 Conjugated (BS-16698R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse.
How should BS-16698R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRE1 (Ser724) Polyclonal Antibody, Cy5.5 Conjugated (BS-16698R-CY5-5)?
KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-16698R-CY5-5)?
Cy5.5 on BS-16698R-CY5-5: excitation / emission 675nm/694nm.