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IRF2BP2 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-16701R-FITC-100uL
Product Name IRF2BP2 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody has displayed cross-reactivity to an unknown protein in the MCF-7 cell line, but not Hela.
Reactivity Human
Predicted Reactivity Cow, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IRF2BP2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 359948
Swiss Prot Q7Z5L9
Subcellular location Nucleus
Synonyms Interferon regulatory factor 2-binding protein 2; IRF-2-binding protein 2; IRF-2BP2; IRF2BP2
Background Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IRF2BP2 Polyclonal Antibody, FITC Conjugated (bs-16701R-FITC) validated for, and at what dilution?

bs-16701R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IRF2BP2 Polyclonal Antibody, FITC Conjugated (bs-16701R-FITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Rabbit.

How should bs-16701R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IRF2BP2 Polyclonal Antibody, FITC Conjugated (bs-16701R-FITC)?

KLH conjugated synthetic peptide derived from human IRF2BP2

What are the excitation and emission wavelengths of the FITC conjugate (bs-16701R-FITC)?

FITC on bs-16701R-FITC: excitation / emission 494nm/518nm.

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