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IRF2BP2 Polyclonal Antibody

Human HeLa lysate 30ug, probed (bs-16701R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:10000 90min in 37˚C.
Human HeLa lysate 30ug, probed (bs-16701R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:10000 90min in 37˚C.
Formalin-fixed and paraffin embedded human kidney labeled with Anti-IRF2BP2 Polyclonal Antibody, Unconjugated (bs-16701R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney labeled with Anti-IRF2BP2 Polyclonal Antibody, Unconjugated (bs-16701R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human

Predicted Reactivity

  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-16701R
Product Name IRF2BP2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Specificity This antibody has displayed cross-reactivity to an unknown protein in the MCF-7 cell line, but not Hela.
Reactivity Human
Predicted Reactivity Cow, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IRF2BP2
Immunogen Range 301-400/587
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 359948
Swiss Prot Q7Z5L9
Subcellular location Nucleus
Synonyms Interferon regulatory factor 2-binding protein 2; IRF-2-binding protein 2; IRF-2BP2; IRF2BP2
Background Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

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