KLHDC8B Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16762R-BF647 |
| Product Name | KLHDC8B Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLHDC8B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 200942 |
| Swiss Prot | Q8IXV7 |
| Subcellular location | Cytoplasm |
| Synonyms | FLJ11302; FP17659; Kelch domain containing 8B; Kelch domain containing protein 8B; Kelch domain-containing protein 8B; KLD8B_HUMAN; Klhdc8b; MGC35097; MGC94736. |
| Background | Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis (PubMed:20107318). Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells (PubMed:22988245, PubMed:23713010). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KLHDC8B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16762R-BF647) validated for, and at what dilution?
bs-16762R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KLHDC8B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16762R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Rabbit.
How should bs-16762R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KLHDC8B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-16762R-BF647)?
KLH conjugated synthetic peptide derived from human KLHDC8B
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-16762R-BF647)?
AbBy Fluor-647 on bs-16762R-BF647: excitation / emission 650nm/665nm.