KLHL18 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16766R-BF680 |
| Product Name | KLHL18 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLHL18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23276 |
| Swiss Prot | O94889 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Kelch-like protein 18; KLHL18; KIAA0795; OK/SW-cl.74 |
| Background | KLHL18 is a 574 amino acid protein that is related to the Drosophila kelch protein, which is required to maintain actin organization in ovarian ring canals. Mutations affecting Kelch function result in failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL18 protein contains six kelch repeats, one BACK (BTB/Kelch associated) domain and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL18 that are produced as a result of alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KLHL18 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-16766R-BF680) validated for, and at what dilution?
bs-16766R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KLHL18 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-16766R-BF680) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Horse.
How should bs-16766R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KLHL18 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-16766R-BF680)?
KLH conjugated synthetic peptide derived from human KLHL18
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-16766R-BF680)?
AbBy Fluor-680 on bs-16766R-BF680: excitation / emission 679nm/702nm.