KLHL18 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16766R-Biotin-100uL |
| Product Name | KLHL18 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Horse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLHL18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 23276 |
| Swiss Prot | O94889 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Kelch-like protein 18; KLHL18; KIAA0795; OK/SW-cl.74 |
| Background | KLHL18 is a 574 amino acid protein that is related to the Drosophila kelch protein, which is required to maintain actin organization in ovarian ring canals. Mutations affecting Kelch function result in failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL18 protein contains six kelch repeats, one BACK (BTB/Kelch associated) domain and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL18 that are produced as a result of alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is KLHL18 Polyclonal Antibody, Biotin Conjugated (bs-16766R-Biotin) validated for, and at what dilution?
bs-16766R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KLHL18 Polyclonal Antibody, Biotin Conjugated (bs-16766R-Biotin) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Horse.
How should bs-16766R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KLHL18 Polyclonal Antibody, Biotin Conjugated (bs-16766R-Biotin)?
KLH conjugated synthetic peptide derived from human KLHL18