KREMEN2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16813R-Cy3 |
| Product Name | KREMEN2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Sheep |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KREMEN2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79412 |
| Swiss Prot | Q8NCW0 |
| Subcellular location | Cell membrane |
| Synonyms | Dickkopf receptor 2; KREM2_HUMAN; KREMEN 2; Kremen protein 2; Kremen protein 2 precursor; Kremen2; Kringle containing protein marking the eye and the nose; Kringle containing transmembrane protein 2; Kringle domain containing transmembrane protein 2; Kringle domain-containing transmembrane protein 2; Kringle-containing protein marking the eye and the nose; KRM 2; KRM2; MGC10791; MGC16709. |
| Background | This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor. A similar protein in mouse functions interacts with with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein forms a ternary membrane complex with DKK1 and the WNT receptor lipoprotein receptor-related protein 6 (LRP6), and induces rapid endocytosis and removal of LRP6 from the plasma membrane. It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KREMEN2 Polyclonal Antibody, Cy3 Conjugated (bs-16813R-Cy3) validated for, and at what dilution?
bs-16813R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KREMEN2 Polyclonal Antibody, Cy3 Conjugated (bs-16813R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Sheep.
How should bs-16813R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KREMEN2 Polyclonal Antibody, Cy3 Conjugated (bs-16813R-Cy3)?
KLH conjugated synthetic peptide derived from human KREMEN2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-16813R-Cy3)?
Cy3 on bs-16813R-Cy3: excitation / emission 512,550nm/570,615nm.