Help

MEK3 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1689R-Cy7
Product Name MEK3 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MKK3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5606
Swiss Prot P46734
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPKK 3; MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; MAP2K3; SKK2
Background Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MEK3 Polyclonal Antibody, Cy7 Conjugated (bs-1689R-Cy7) validated for, and at what dilution?

bs-1689R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MEK3 Polyclonal Antibody, Cy7 Conjugated (bs-1689R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1689R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MEK3 Polyclonal Antibody, Cy7 Conjugated (bs-1689R-Cy7)?

KLH conjugated synthetic peptide derived from human MKK3

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1689R-Cy7)?

Cy7 on bs-1689R-Cy7: excitation / emission 743nm/767nm.