MEK3 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: rat skeletal muscle lysates probed with MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: rat skeletal muscle lysates probed with MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1689R
Product Name	MEK3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MKK3
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5606
Swiss Prot	P46734
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPKK 3; MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; MAP2K3; SKK2
Background	Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200